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GS FLX+ System

Now delivering sequencing reads up to 1,000 bp in length!

The GS FLX+ System features the unique combination of long reads, exceptional accuracy and high-throughput, making the system well suited for larger genomic projects. The GS FLX System has been at the heart of key breakthrough genomic discoveries and over 1,000 peer-reviewed publications to date.

Advancements in sequencing chemistry, instrumentation and software offer the latest improvements in GS FLX System performance, with read lengths up to 1 kb. Available as an on-site instrument upgrade or new instrument, the GS FLX+ System is designed for use with both the new long-read Sequencing Kit XL+ and existing Sequencing Kit XLR70.

  • True capillary sequencing-like read lengths: Continuous development of the GS FLX Titanium chemistry now offers read lengths up to 1 kb
  • Flexible sequencing formats: Broad selection of gaskets and Multiplex Identifiers (MIDs) enables efficient use of sequencing runs
  • Point-and-click data analysis: Included GS Data Analysis Software for de novo assembly, reference mapping and amplicon variant analysis.
  • High-throughput sequencing for a wide range of applications:

GS FLX+ Instrument: The fluidics subsystem facilitates accurate dispensing, flow and removal. The optics subsystem consists of a CCD camera which captures the light emitted in the PicoTiterPlate device wells during the sequencing. The computer subsystem controls the fluidics and optics subsystems and processes the digital images sent by the camera to extract the DNA sequence information.

GS FLX+ System
Sequencing Kit New! GS FLX Titanium XL+ GS FLX Titanium XLR70
Read Length Up to 1,000 bp Up to 600 bp
Mode Read Length 700 bp 450 bp
Throughput Profile - 85% of total bases from reads >500 bp
- 45% of total bases from reads >700 bp
- 85% of total bases from reads > 300 bp
- 20% of total bases from reads > 500 bp
Typical Throughput 700 Mb 450 Mb
Reads per Run ~1,000,000 shotgun ~1,000,000 shotgun,
~700,000 amplicon
Consensus Accuracy* 99.997% 99.995%
Run Time 23 hours 10 hours
Sample Input gDNA or cDNA gDNA, cDNA, or amplicons (PCR products)
Multiplexing Multiplex Identifiers (MIDs): 132
Gaskets: 2, 4, 8, 16 regions
Computing GS FLX+ Computing Station available
Dimensions Upper assembly
74.3 cm 69.8 cm 36.1 cm (29.25 27.5 14.2)
(W D H), incl. monitor 82.5 cm (32.5) H
Lower assembly
75.2 cm 90.8 cm 92.7 cm (29.62 35.75 36.5)
(W D H)
Weight 532 lbs (242 kg)
Power Supply 120 VAC 50/60 Hz 1250 VA
230 VAC 50/60 Hz 1250 VA

Actual results depend on specific sample and genomic characteristics.
*Consensus accuracy at 15x coverage.

Read length distribution of 1,293,269 reads from a GS FLX+ run of the parakeet genome. Mode read length is 793 bp.

Product Name Pack Size Catalog Number
GS FLX Titanium Rapid Library Preparation Kit 1 Kit (for 12 library preparations) 05608228001
GS FLX Titanium Rapid Library MID Adaptors 1 Kit (for 96 library preparations) 05619211001
GS FLX Titanium Library Paired End Adaptors 1 kit (for 10 assays) 05463343001
GS FLX Titanium General Library Preparation Kit 1 kit (for 10 library preparations) 05233747001
GS FLX Titanium LV emPCR Kit (Lib-L) 1 kit (for 2 large volume emulsion amplification reactions) 05618428001
GS FLX Titanium MV emPCR Kit (Lib-L) 1 kit (for 8 medium volume emulsion amplification reactions) 05618436001
GS FLX Titanium SV emPCR Kit (Lib-L) 1 kit (for 16 small volume emulsion amplification reactions) 05618444001
GS FLX Titanium LV emPCR Kit (Lib-A) 1 kit (for 2 large volume emulsion amplification reactions) 05619114001
GS FLX Titanium MV emPCR Kit (Lib-A) 1 kit (for 8 medium volume emulsion amplification reactions) 05619149001
GS FLX Titanium SV emPCR Kit (Lib-A) 1 kit (for 32 small volume emulsion amplification reactions) 05619165001
GS FLX Titanium emPCR Breaking Kit LV/MV 1 kit (for 12 LV emulsion-breaking setups) 05233658001
GS FLX Titanium emPCR Filters SV 1 kit (64 filters) 05233674001
GS FLX Titanium Sequencing Kit XLR70 1 kit (for one sequencing run) 05233526001
New! GS FLX Titanium Sequencing Kit XL+ 1 kit (for one sequencing run) 06380565001
GS FLX Titanium PicoTiterPlate Kit 70x75 1 plate (with accessories) 05233682001
GS FLX Titanium Control Bead Kit 1 kit (for one sequencing run) 05470129001
GS FLX Maintenance Wash Kit 1 kit 04932358001
Instruments & Hardware
GS FLX+ Instrument Instrument 06372279001
GS FLX+ Instrument Upgrade Kit Kit 05872430001
GS FLX+ Computing Station Instrument 06388779001
GS FLX+ Computing Station Monitor Monitor 06388787001

Literature

Videos

Genome Sequencer FLX Multimedia Presentation: Watch this short flash presentation describing the GS FLX Instrument and an overview of the technology involved in a sequencing run.
View Presentation


For manuals and other system documentation, visit the customer-accessible my454 site.